Michael Koob, Ph.D.

Contact Information Phone: 612-626-4521 612-626-6516 Fax: Email: koobx001@umn.edu Address: MMC 609 Mayo 8609 420 Delaware Minneapolis, MN 55455

Assistant Professor

Educational Background

• North Dakota State University, Fargo (1986), B.S. (Chemistry)
• University of Wisconsin - Madison (1990), Ph.D. (Molecular and Cellular Biology)
• University of Wisconsin - Madison (1991), Postdoctoral Associate
• University of Wisconsin - Madison (1994), Assistant Researcher

Publications

• Yoon, Y.G., Haug, C., and Koob, M.D. Interspecies mitochondrial fusion between mouse and human mitochondria is rapid and efficient. Mitochondrion, 7, 223-229, 2007.
• Aromolaran, K.A., Benzow, K.A., Koob, M.D., and Piedras-Renteria, E.S. The Kelch-like protein 1 modulates P/Q-type current density. Neuroscience, 145: 841-850, 2007.
• He Y, Zu T, Benzow KA, Orr HT, Clark HB, Koob MD. Targeted deletion of a single Sca8 ataxia locus allele in mice causes abnormal gait, progressive loss of motor coordination, and Purkinje cell dendritic deficits. J Neurosci. 26: 9975-82, 2006.
• Yoon, Y.G. and Koob, M.D. Transformation of mammalian mitochondria by bacterial conjugation, Nucleic Acids Research 33, e139, 2005.
• Mutsuddi, M, Marshall, C.M., Benzow, K.A., Koob, M.D., and Rebay, I. The Spinocerebellar Ataxia 8 non-coding RNA causes neurodegeneration and associates with Staufen in Drosophila. Current Biology 14: 302-308, 2004.
• Yoon, Y.G., and Koob, M.D. Efficient cloning and engineering of entire mitochondrial genomes in Escherichia coli and transfer into transcriptionally active mitochondria. Nucleic Acids Research 31, 1407-1415. 2003.
• Benzow, K.A., and Koob, M.D. The KLHL1-antisense transcript (KLHL1AS) is evolutionarily conserved. Mammalian Genome 13, 134-141, 2002.
• Yu, B.J., Sung, B.H., Koob, M.D., Lee, C.H., Lee J.H., Lee, W.K., Kim, M.S., and Kim, S.C. Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision system. Nature Biotech. 20, 1018-1023, 2002.
• Benzow, K.A., Koob, M.D., Condie, A., Catovsky, D., Matutes, E., Yuille, M.R., Houlston, R.S. Instability of CAG-trinucleotide repeats in chronic lymphocytic leukemia. Leukemia & Lymphoma 43, 1987-1990, 2002.

Invited Lectures

• January 15, 2007. Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, MN. "Re-engineering the mammalian mitochondrial genome."
• January 31, 2007. Department of Neuroscience, Albert Einstein College of Medicine, New York, NY. “Partial loss of Klhl1 expression leads to a progressive loss of motor coordination: a possible underlying molecular cause of Spinocerebellar Ataxia type 8 (SCA8)”
• March 15-18, 2006: Spring Brain Conference, Sedona, AZ “Detailed characterization of the Kelch-like 1 (KLHL1) sense/antisense locus in tissue culture and animal systems.” Invited plenary session presentation.
• September 14, 2005: Physiology Department Seminar, Loyola Medical School, Chicago, IL “Untangling the complexities of the SCA8 ataxia locus.”
• October 27, 2005: American Society of Human Genetics annual meeting, Salt Lake City, Utah
• November 17, 2004, “Modeling Human Mitochondrial Diseases in the Mouse". Department of Laboratory Medicine and Pathology Grand Rounds
• February 5, 2003, "Making Sense of antisense: molecular analyses of the sense and antisense genes of the SCA8 ataxia locus using cell and mouse models." Department of Laboratory Medicine and Pathology Grand Rounds
• May 15, 2003, “Re-engineering the mitochondrial genome.” Genetic Mechanisms of Cancer/ Institute of Human Genetics Research Seminar
• October 18, 2002, “Cloning and engineering of entire mitochondrial genomes in Escherichia coli and transfer into isolated mitochondria.” Baltimore, MD American Society of Human Genetics platform presentation 

Presentations

• Annual Meeting of the American Society of Human Genetics
  • “Cloning and engineering of entire mitochondrial genomes in Escherichia coli and transfer into isolated mitochondria.” Young Yoon, Michael Koob, 2002 (platform presentation).
  • “KLHL1, the gene expressed from the SCA8 locus, and KLHL4 evolved from the same orthologous gene in fish.” You Mie Kim, Michael Koob, 2002.
  • “Transmitochondrial yeast strains containing the complete mouse mitochondrial genome: a new in vivo model system for analyzing mammalian nuclear-mitochondrial interactions.” Young Yoon, Michael Koob, 2003.
  • “Targeted deletion of the Sca8 ataxia locus in mice causes abnormal gait, progressive loss of motor coordination and Purkinje cell dendritic deficits.” He, Y., K.E. Benzow, H.B. Clark, M.D. Koob, 2005.
  • “Using a Drosophila model to characterize Spinocerebellar Ataxia 8 (SCA8) induced neurodegeneration” M Mutsuddi, Kellie Benzow, JT Littleton, Michael Koob & Ilaria Rebay
• Society for Neuroscience
  • “On the co-incidence of SCA6 and SCA8: the Kelch-like 1 protein does not modify the function of the neuronal P/Q-type calcium channel.” Kelly A. Savoie, Kellie A. Benzow, Michael D. Koob, Erika S. Piedras-Rentería, 2004.
  • “The actin-binding protein Kelch-like 1 modulates T-type calcium current density.” Savoie, K.A, Benzow, K.A, Cribbs, L.L., Koob, M.D., Piedras-Rentería E.S. 2006.
  • “Untangling the interactions between the Kelch-Like1 (KLHL1) sense and antisense transcripts.” Benzow, K.A., and Koob, M.D. 2006.
  • “Detailed characterization of the Kelch-like 1 (KLHL1) sense/antisense locus in tissue culture and animal systems.” He, Y., K.E. Benzow, H.B. Clark, M.D. Koob. 2006.