Stephen Hecht, Ph.D.

Contact Information Phone: 612-624-7604 Fax: Email: hecht002@umn.edu Address: MMC 806 Mayo 8806 420 Delaware Minneapolis, MN 55455 Dr. Hecht @ UMN College of Pharmacy

Professor

Educational Background

• Duke University, North Carolina (1964), B.S. (Chemistry)
• Massachusetts Institute of Technology, Massachusetts (1968), Ph.D. (Organic Chemistry)

Professional Background

• Wallin Professor of Cancer Prevention, University of Minnesota, Cancer Center, Minneapolis, MN, 1996 - present
• Director of Research, American Health Foundation, Naylor Dana Institute for Disease Prevention, Valhalla, NY, 1987 - 1996
• National Research Council Fellow, Agricultural Research Service, United States Department of Agriculture, Philadelphia, PA, 1971 - 1973
• Assistant Professor of Chemistry, Haverford College, Haverford, PA, 1969 - 1971
• Chief, Division of Chemical Carcinogenesis, American Health Foundation, Naylor Dana Institute for Disease Prevention, Valhalla, NY, 1980 - 1996
• Head, Section of Organic Chemistry, Division of Environmental Carcinogenesis, American Health Foundation, Naylor Dana Institute for Disease Prevention, Valhalla, NY, 1973 - 1980

Professional Honors

• Phi Beta Kappa
• National Research Council Research Fellow, 1971 - 1973
• Research Career Development Award, National Cancer Institute, 1975 - 1980
• Outstanding Investigator Grant, National Cancer Institute, 1987 - 2001
• Cancer Research Covers, March 1, 1988; February 15, 1993
• Endowed Chair in Carcinogenesis and Chemoprevention, American Health Foundation, 1992 - 1996
• Wallin Chair in Cancer Prevention, University of Minnesota, 1996 - present
• American Cancer Society Research Professor, 1999 - present
• Alton Ochsner Award Relating Smoking and Health, 2001

Community and University Service

• Surgeon General’s Report, Passive Smoking and Health, Contributor, 2002
• Lung Cancer Progress Review Group, National Cancer Institute, 2001
• American Cancer Society, Chair, 2001, Peer Review Committee on Carcinogenesis, Nutrition, and the Environment, 1998 - 2001
• National Center for Toxicological Research, FDA, Science Advisory Board, 1998
• National Toxicology Program, Board of Scientific Counselors, 1997 - 2001
• Division of Chemical Toxicology, American Chemical Society, Chair, 1999-2000; Chair-elect, 1997-1998; Program Chair, 1996
• National Cancer Institute, Board of Scientific Counselors, Division of Cancer Etiology, 1989 - 1995
• National Institutes of Health, Chemical Pathology Study Section; Ad hoc, 1998, 1981 - 1985
• American Institute for Cancer Research, Grants Review Panel, 1984 - 1987
• Health Effects Institute, Health Research Committee, 1992 - 1996
• Oregon State University, External Advisory Committee, Environmental Health Sciences Center, 1996 - 2001
• Massachusetts Institute of Technology, Corporation Visiting Comm., Div of Bioengineering and Environmental Health, 2000
• Vanderbilt University, Advisory Group, Center in Molecular Toxicology; Chairman, 1995-1997, 1991 - 1997
• Editorial Boards:, Cancer Research; Cancer Epidemiology, Biomarkers & Prevention; Cancer Letters; Carcinogenesis; Lung Cancer Program Comm., Amer. Assoc. for Cancer Research, 1983, 1990, 1993, 1997, 2000; Session chair, 1984, 1986, 1988, 1991, 2000
• National Cancer Institute, Upper Aerodigestive Cancer Working Group, 1986 - 1989
• Carcinogenesis Technical Support Comm., Surgeon General's Report: Health Consequences of Using Smokeless Tobacco, 1986
• Organizing Committee, NIH Consensus Development Conference on the Health Implications of Smokeless Tobacco, 1986
• Chemical Carcinogenesis, Principal Lecturer, American Chemical Society Short Course, 1979 - 1983
• Consultant, WHO, IARC, Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans, 1984
• Thesis Committees: Rutgers Univ, Massachusetts Institute of Technology, Columbia Univ, Univ of Minnesota

Publications

• Joseph AM, Hecht SS, Murphy SE, Lando H, Carmella SG, Han S, Gross M, Bliss R, Le CT, and Hatsukami DK. Smoking reduction fails to improve clinical and biological markers of cardiac disease: a randomized controlled trial. Nicotine. Tobacco Res.., in press, 2008.
• Hecht SS, Carmella SG, Edmonds A, Murphy SE, Stepanov I, Luo X, and Hatsukami DK. Exposure to nicotine and a tobacco-specific carcinogen increase with duration of use of smokeless tobacco. Tobacco Control, in press, 2008.
• Stepanov I and Hecht SS. Detection and quantitation of N′-nitrosonornicotine in human toenails by liquid chromatography-electrospray ionization-tandem mass spectrometry. Cancer Epidemiol, Biomarkers, & Prev.,in press, 2008.
• Hecht SS, Villalta PW, and Hochalter JB. Analysis of phenanthrene diol epoxide mercapturic acid detoxification products in human urine: relevance to molecular epidemiology studies of glutathione-S-transferase polymorphisms. Carcinogenesis, in press, 2008.
• Hecht SS, Carmella SG, Stepanov I, Jensen J, Anderson A, and Hatsukami D. Metabolism of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone to its biomarker total NNAL in smokeless tobacco users. Cancer Epidemiol, Biomarkers, & Prev., in press, 2008.
• Balbo S, Hashibe M, Gundy S, Brennan P, Canova C, Simonato L, Merletti F, Richiardi L, Agudo A, Castellsague X, Znaor A, Talamini R, Bencko V, Holcatova I, Wang M, Hecht SS, and Boffetta P. N2-Ethyldeoxyguanosine as a potential biomarker for assessing effects of alcohol consumption on DNA. Cancer Epidemiol, Biomarkers, & Prev., submitted, 2008.
• Wang M, Cheng G, Villalta PW, Hecht SS: Development of Liquid Chromatograpy Electrospray Ionization Tandem Mass Spectrometry Methods for Analysis of DNA Adducts of Formaldehyde and Their Application to Rats Treated with N-Nitrosodimethylamine or 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone, Chem Res Toxicol 2008, 21:555
• Kassie F, Anderson LB, Higgins L, Pan Y, Matise I, Negia M, Upadhyaya P, Wang M, Hecht SS: Chemopreventive agents modulate the protein expression profile of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone plus benzo[a]pyrene-induced lung tumors in A/J mice, Carcinogenesis epub ahead of print Jan 12, 2008.
• Hatsukami DK, Ebbert JO, Edmonds A, Li C, Lin H, Le C, Hecht SS: Smokeless tobacco reduction: Preliminary study of tobacco-free snuff versus no snuff, Nicotine Tob Res 2008, 10:77-85
• Cheng G, Wang M, Upadhyaya P, Villalta PW, Hecht SS: Formation of Formaldehyde Adducts in the Reactions of DNA and Deoxyribonucleosides with α-Acetates of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), and N-Nitrosodimethylamine (NDMA), Chem Res Toxicol epub ahead of print Jan 19, 2008.
• Hatsukami DK, Mooney M, Babb D, Murphy S, and Hecht SS. Effects of high dose transdermal nicotine replacement in cigarette smokers. Pharmacology, Biochemistry, and Behavior, 86: 132-139, 2007.
• Johnson TE, Kassie F, O’Sullivan G, Negia M, Hanson TE, Upadhyaya P, Ruvolo PP, Hecht SS, and Xing C. Chemopreventive effect of kava on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone plus benzo[a]pyrene-induced lung tumorigenesis in A/J mice. Cancer Res., submitted, 2007.
• Zhang S, Villalta PW, Wang M, Hecht SS: Detection and quantitation of acrolein-derived 1,N2-propanodeoxyguanosine adducts in human lung by liquid chromatography-electrospray ionization-tandem mass spectrometry, Chem Res Toxicol 2007, 20:565-571
• Wang M, Lao Y, Cheng G, Shi Y, Villalta PW, Nishikawa A, Hecht SS: Analysis of adducts in hepatic DNA of rats treated with N-nitrosopyrrolidine, Chem Res Toxicol 2007, 20:634-640
• Wang M, Lao Y, Cheng G, Shi Y, Villalta PW, Hecht SS: Identification of adducts formed in the reaction of alpha-acetoxy-N-nitrosopyrrolidine with deoxyribonucleosides and DNA, Chem Res Toxicol 2007, 20:625-633
• Wang M, Cheng G, Villalta PW, Hecht SS: Development of liquid chromatography electrospray ionization tandem mass spectrometry methods for analysis of DNA adducts of formaldehyde and their application to rats treated with N-nitrosodimethylamine or 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, Chem Res Toxicol 2007, 20:1141-1148
• Stepanov I, Hecht SS, Lindgren B, Jacob P, 3rd, Wilson M, Benowitz NL: Relationship of human toenail nicotine, cotinine, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol to levels of these biomarkers in plasma and urine, Cancer Epidemiol Biomarkers Prev 2007, 16:1382-1386
• Stark MJ, Rohde K, Maher JE, Pizacani BA, Dent CW, Bard R, Carmella SG, Benoit AR, Thomson NM, Hecht SS: The impact of clean indoor air exemptions and preemption policies on the prevalence of a tobacco-specific lung carcinogen among nonsmoking bar and restaurant workers, Am J Public Health 2007, 97:1457-1463
• Spector LG, Hecht SS, Ognjanovic S, Carmella SG, Ross JA: Detection of cotinine in newborn dried blood spots, Cancer Epidemiol Biomarkers Prev 2007, 16:1902-1905
• Porubin D, Hecht SS, Li ZZ, Gonta M, Stepanov I: Endogenous formation of N'-nitrosonornicotine in F344 rats in the presence of some antioxidants and grape seed extract, J Agric Food Chem 2007, 55:7199-7204
• Mendoza-Baumgart MI, Tulunay OE, Hecht SS, Zhang Y, Murphy S, Le C, Jensen J, Hatsukami DK: Pilot study on lower nitrosamine smokeless tobacco products compared with medicinal nicotine, Nicotine Tob Res 2007, 9:1309-1323
• McNiel EA, Carmella SG, Heath LA, Bliss RL, Le KA, Hecht SS: Urinary biomarkers to assess exposure of cats to environmental tobacco smoke, Am J Vet Res 2007, 68:349-353
• Lubin JH, Caporaso N, Hatsukami DK, Joseph AM, Hecht SS: The association of a tobacco-specific biomarker and cigarette consumption and its dependence on host characteristics, Cancer Epidemiol Biomarkers Prev 2007, 16:1852-1857
• Liu X, Moody EC, Hecht SS, Sturla SJ: Deoxygenated phosphorothioate inositol phosphate analogs: Synthesis, phosphatase stability, and binding affinity, Bioorg Med Chem, epub ahead of print Oct 14, 2007.
• Lao Y, Yu N, Kassie F, Villalta PW, Hecht SS: Analysis of pyridyloxobutyl DNA adducts in F344 rats chronically treated with (R)- and (S)-N'-nitrosonornicotine, Chem Res Toxicol 2007, 20:246-256
• Lao Y, Yu N, Kassie F, Villalta PW, Hecht SS: Formation and accumulation of pyridyloxobutyl DNA adducts in F344 rats chronically treated with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and enantiomers of its metabolite, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, Chem Res Toxicol 2007, 20:235-245
• Kassie F, Anderson LB, Scherber R, Yu N, Lahti D, Upadhyaya P, Hecht SS: Indole-3-carbinol inhibits 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone plus benzo(a)pyrene-induced lung tumorigenesis in A/J mice and modulates carcinogen-induced alterations in protein levels, Cancer Res 2007, 67:6502-6511
• Hecht SS, Han S, Kenney PM, Wang M, Lindgren B, Wang Y, Lao Y, Hochalter JB, Upadhyaya P: Investigation of the reaction of myosmine with sodium nitrite in vitro and in rats, Chem Res Toxicol 2007, 20:543-549
• Hecht SS, Carmella SG, Murphy SE, Riley WT, Le C, Luo X, Mooney M, Hatsukami DK: Similar exposure to a tobacco-specific carcinogen in smokeless tobacco users and cigarette smokers, Cancer Epidemiol Biomarkers Prev 2007, 16:1567-1572
• Hatsukami DK, Ebbert JO, Anderson A, Lin H, Le C, Hecht SS: Smokeless tobacco brand switching: a means to reduce toxicant exposure?, Drug Alcohol Depend 2007, 87:217-224
• Chen L, Wang M, Villalta PW, Luo X, Feuer R, Jensen J, Hatsukami DK, Hecht SS: Quantitation of an acetaldehyde adduct in human leukocyte DNA and the effect of smoking cessation, Chem Res Toxicol 2007, 20:108-113
• Chen L, Wang M, Villalta PW, Hecht SS: Liquid chromatography-electrospray ionization tandem mass spectrometry analysis of 7-ethylguanine in human liver DNA, Chem Res Toxicol 2007, 20:1498-1502
• Carmella SG, Chen M, Zhang Y, Zhang S, Hatsukami DK, Hecht SS: Quantitation of acrolein-derived (3-hydroxypropyl)mercapturic acid in human urine by liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry: effects of cigarette smoking, Chem Res Toxicol 2007, 20:986-990
• Zhang S, Villalta PW, Wang M, Hecht SS: Analysis of crotonaldehyde- and acetaldehyde-derived 1,n(2)-propanodeoxyguanosine adducts in DNA from human tissues using liquid chromatography electrospray ionization tandem mass spectrometry, Chem Res Toxicol 2006, 19:1386-1392
• Wang M, Yu N, Chen L, Villalta PW, Hochalter JB, Hecht SS: Identification of an acetaldehyde adduct in human liver DNA and quantitation as N2-ethyldeoxyguanosine, Chem Res Toxicol 2006, 19:319-324
• Upadhyaya P, Rao P, Hochalter JB, Li ZZ, Villalta PW, Hecht SS: Quantitation of N-acetyl-S-(9,10-dihydro-9-hydroxy-10-phenanthryl)-L-cysteine in human urine: comparison with glutathione-S-transferase genotypes in smokers, Chem Res Toxicol 2006, 19:1234-1240
• Upadhyaya P, McIntee EJ, Villalta PW, Hecht SS: Identification of adducts formed in the reaction of 5'-acetoxy-N'-nitrosonornicotine with deoxyguanosine and DNA, Chem Res Toxicol 2006, 19:426-435
• Stepanov I, Jensen J, Hatsukami D, Hecht SS: Tobacco-specific nitrosamines in new tobacco products, Nicotine Tob Res 2006, 8:309-313
• Stepanov I, Hecht SS, Duca G, Mardari I: Uptake of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone by Moldovan children, Cancer Epidemiol Biomarkers Prev 2006, 15:7-11
• Stepanov I, Feuer R, Jensen J, Hatsukami D, Hecht SS: Mass spectrometric quantitation of nicotine, cotinine, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human toenails, Cancer Epidemiol Biomarkers Prev 2006, 15:2378-2383
• Stein S, Lao Y, Yang IY, Hecht SS, Moriya M: Genotoxicity of acetaldehyde- and crotonaldehyde-induced 1,N2-propanodeoxyguanosine DNA adducts in human cells, Mutat Res 2006, 608:1-7
• Liu X, Lao Y, Yang IY, Hecht SS, Moriya M: Replication-coupled repair of crotonaldehyde/acetaldehyde-induced guanine-guanine interstrand cross-links and their mutagenicity, Biochemistry 2006, 45:12898-12905
• Lao Y, Villalta PW, Sturla SJ, Wang M, Hecht SS: Quantitation of pyridyloxobutyl DNA adducts of tobacco-specific nitrosamines in rat tissue DNA by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry, Chem Res Toxicol 2006, 19:674-682
• Hecht SS, Huang C, Stoner GD, Li J, Kenney PM, Sturla SJ, Carmella SG: Identification of cyanidin glycosides as constituents of freeze-dried black raspberries which inhibit anti-benzo[a]pyrene-7,8-diol-9,10-epoxide induced NFkappaB and AP-1 activity, Carcinogenesis 2006, 27:1617-1626
• Hecht SS, Carmella SG, Yoder A, Chen M, Li ZZ, Le C, Dayton R, Jensen J, Hatsukami DK: Comparison of polymorphisms in genes involved in polycyclic aromatic hydrocarbon metabolism with urinary phenanthrene metabolite ratios in smokers, Cancer Epidemiol Biomarkers Prev 2006, 15:1805-1811
• Hecht SS, Carmella SG, Le KA, Murphy SE, Boettcher AJ, Le C, Koopmeiners J, An L, Hennrikus DJ: 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronides in the urine of infants exposed to environmental tobacco smoke, Cancer Epidemiol Biomarkers Prev 2006, 15:988-992
• Hatsukami DK, Le CT, Zhang Y, Joseph AM, Mooney ME, Carmella SG, Hecht SS: Toxicant exposure in cigarette reducers versus light smokers, Cancer Epidemiol Biomarkers Prev 2006, 15:2355-2358
• Derave W, Vanden Eede E, Hespel P, Carmella SG, Hecht SS: Oral creatine supplementation in humans does not elevate urinary excretion of the carcinogen N-nitrososarcosine, Nutrition 2006, 22:332-333
• Carmella SG, Yoder A, Hecht SS: Combined analysis of r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4-tetrahydrophenanthrene and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in smokers' plasma, Cancer Epidemiol Biomarkers Prev 2006, 15:1490-1494
• Wong HL, Zhang X, Zhang QY, Gu J, Ding X, Hecht SS, Murphy SE: Metabolic activation of the tobacco carcinogen 4-(methylnitrosamino)-(3-pyridyl)-1-butanone by cytochrome P450 2A13 in human fetal nasal microsomes, Chem Res Toxicol 2005, 18:913-918
• Wong HL, Murphy SE, Hecht SS: Cytochrome P450 2A-catalyzed metabolic activation of structurally similar carcinogenic nitrosamines: N'-nitrosonornicotine enantiomers, N-nitrosopiperidine, and N-nitrosopyrrolidine, Chem Res Toxicol 2005, 18:61-69
• Rosen CJ, Fritz VA, Hecht SS, Gardner GM, Carmella SG, and Kenney PM. Cabbage yield and glucosinolate concentrations as affected by nitrogen and sulfur fertility. Hort. Science, 40: 1493-1498, 2005
• Tulunay OE, Hecht SS, Carmella SG, Zhang Y, Lemmonds C, Murphy S, Hatsukami DK: Urinary metabolites of a tobacco-specific lung carcinogen in nonsmoking hospitality workers, Cancer Epidemiol Biomarkers Prev 2005, 14:1283-1286
• Sturla SJ, Scott J, Lao Y, Hecht SS, Villalta PW: Mass spectrometric analysis of relative levels of pyridyloxobutylation adducts formed in the reaction of DNA with a chemically activated form of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, Chem Res Toxicol 2005, 18:1048-1055
• Stepanov I, Hecht SS, Ramakrishnan S, Gupta PC: Tobacco-specific nitrosamines in smokeless tobacco products marketed in India, Int J Cancer 2005, 116:16-19
• Stepanov I, Hecht SS, Mirvish SS, Gonta M: Comparative analysis of tobacco-specific nitrosamines and total N-nitroso compounds in moldovan cigarette tobacco, J Agric Food Chem 2005, 53:8082-8086
• Stepanov I, Hecht SS: Tobacco-specific nitrosamines and their pyridine-N-glucuronides in the urine of smokers and smokeless tobacco users, Cancer Epidemiol Biomarkers Prev 2005, 14:885-891
• Lemmonds CA, Hecht SS, Jensen JA, Murphy SE, Carmella SG, Zhang Y, Hatsukami DK: Smokeless tobacco topography and toxin exposure, Nicotine Tob Res 2005, 7:469-474
• Lao Y, Hecht SS: Synthesis and properties of an acetaldehyde-derived oligonucleotide interstrand cross-link, Chem Res Toxicol 2005, 18:711-721
• Kim JY, Hecht SS, Mukherjee S, Carmella SG, Rodrigues EG, Christiani DC: A urinary metabolite of phenanthrene as a biomarker of polycyclic aromatic hydrocarbon metabolic activation in workers exposed to residual oil fly ash, Cancer Epidemiol Biomarkers Prev 2005, 14:687-692
• Kensler TW, Chen JG, Egner PA, Fahey JW, Jacobson LP, Stephenson KK, Ye L, Coady JL, Wang JB, Wu Y, Sun Y, Zhang QN, Zhang BC, Zhu YR, Qian GS, Carmella SG, Hecht SS, Benning L, Gange SJ, Groopman JD, Talalay P: Effects of glucosinolate-rich broccoli sprouts on urinary levels of aflatoxin-DNA adducts and phenanthrene tetraols in a randomized clinical trial in He Zuo township, Qidong, People's Republic of China, Cancer Epidemiol Biomarkers Prev 2005, 14:2605-2613
• Kelley MJ, Glaser EM, Herndon JE, 2nd, Becker F, Bhagat R, Zhang YJ, Santella RM, Carmella SG, Hecht SS, Gallot L, Schilder L, Crowell JA, Perloff M, Folz RJ, Bergan RC: Safety and efficacy of weekly oral oltipraz in chronic smokers, Cancer Epidemiol Biomarkers Prev 2005, 14:892-899
• Joseph AM, Hecht SS, Murphy SE, Carmella SG, Le CT, Zhang Y, Han S, Hatsukami DK: Relationships between cigarette consumption and biomarkers of tobacco toxin exposure, Cancer Epidemiol Biomarkers Prev 2005, 14:2963-2968
• Jalas JR, Hecht SS, Murphy SE: Cytochrome P450 enzymes as catalysts of metabolism of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, a tobacco specific carcinogen, Chem Res Toxicol 2005, 18:95-110
• Hecht SS, Murphy SE, Carmella SG, Li S, Jensen J, Le C, Joseph AM, Hatsukami DK: Similar uptake of lung carcinogens by smokers of regular, light, and ultralight cigarettes, Cancer Epidemiol Biomarkers Prev 2005, 14:693-698
• Hecht SS, Chen M, Yoder A, Jensen J, Hatsukami D, Le C, Carmella SG: Longitudinal study of urinary phenanthrene metabolite ratios: effect of smoking on the diol epoxide pathway, Cancer Epidemiol Biomarkers Prev 2005, 14:2969-2974
• Conaway CC, Wang CX, Pittman B, Yang YM, Schwartz JE, Tian D, McIntee EJ, Hecht SS, Chung FL: Phenethyl isothiocyanate and sulforaphane and their N-acetylcysteine conjugates inhibit malignant progression of lung adenomas induced by tobacco carcinogens in A/J mice, Cancer Res 2005, 65:8548-8557
• Carmella SG, Han S, Villalta PW, Hecht SS: Analysis of total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in smokers' blood, Cancer Epidemiol Biomarkers Prev 2005, 14:2669-2672
• Zimmerman CL, Wu Z, Upadhyaya P, Hecht SS: Stereoselective metabolism and tissue retention in rats of the individual enantiomers of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), metabolites of the tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), Carcinogenesis 2004, 25:1237-1242
• Wogan GN, Hecht SS, Felton JS, Conney AH, Loeb LA: Environmental and chemical carcinogenesis, Semin Cancer Biol 2004, 14:473-486
• Sexton K, Adgate JL, Church TR, Hecht SS, Ramachandran G, Greaves IA, Fredrickson AL, Ryan AD, Carmella SG, Geisser MS: Children's exposure to environmental tobacco smoke: using diverse exposure metrics to document ethnic/racial differences, Environ Health Perspect 2004, 112:392-397
• Murphy SE, Link CA, Jensen J, Le C, Puumala SS, Hecht SS, Carmella SG, Losey L, Hatsukami DK: A comparison of urinary biomarkers of tobacco and carcinogen exposure in smokers, Cancer Epidemiol Biomarkers Prev 2004, 13:1617-1623
• Jalas JR, Seetharaman M, Hecht SS, Murphy SE: Molecular modelling of CYP2A enzymes: application to metabolism of the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), Xenobiotica 2004, 34:515-533
• Hughes JR, Hecht SS, Carmella SG, Murphy SE, Callas P: Smoking behaviour and toxin exposure during six weeks use of a potential reduced exposure product: Omni, Tob Control 2004, 13:175-179
• Hecht SS, Villalta PW, Sturla SJ, Cheng G, Yu N, Upadhyaya P, Wang M: Identification of O2-substituted pyrimidine adducts formed in reactions of 4-(acetoxymethylnitrosamino)- 1-(3-pyridyl)-1-butanone and 4-(acetoxymethylnitros- amino)-1-(3-pyridyl)-1-butanol with DNA, Chem Res Toxicol 2004, 17:588-597
• Hecht SS, Murphy SE, Carmella SG, Zimmerman CL, Losey L, Kramarczuk I, Roe MR, Puumala SS, Li YS, Le C, Jensen J, Hatsukami DK: Effects of reduced cigarette smoking on the uptake of a tobacco-specific lung carcinogen, J Natl Cancer Inst 2004, 96:107-115
• Hecht SS, Carmella SG, Le KA, Murphy SE, Li YS, Le C, Jensen J, Hatsukami DK: Effects of reduced cigarette smoking on levels of 1-hydroxypyrene in urine, Cancer Epidemiol Biomarkers Prev 2004, 13:834-842
• Hecht SS, Carmella SG, Kenney PM, Low SH, Arakawa K, Yu MC: Effects of cruciferous vegetable consumption on urinary metabolites of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in singapore chinese, Cancer Epidemiol Biomarkers Prev 2004, 13:997-1004
• Hatsukami DK, Lemmonds C, Zhang Y, Murphy SE, Le C, Carmella SG, Hecht SS: Evaluation of carcinogen exposure in people who used "reduced exposure" tobacco products, J Natl Cancer Inst 2004, 96:844-852
• Carmella SG, Le KA, Hecht SS: Improved method for determination of 1-hydroxypyrene in human urine, Cancer Epidemiol Biomarkers Prev 2004, 13:1261-1264
• Carmella SG, Chen M, Yagi H, Jerina DM, Hecht SS: Analysis of phenanthrols in human urine by gas chromatography-mass spectrometry: potential use in carcinogen metabolite phenotyping, Cancer Epidemiol Biomarkers Prev 2004, 13:2167-2174
• Wong HL, Murphy SE, Wang M, Hecht SS: Comparative metabolism of N-nitrosopiperidine and N-nitrosopyrrolidine by rat liver and esophageal microsomes and cytochrome P450 2A3, Carcinogenesis 2003, 24:291-300
• Wong HL, Murphy SE, Hecht SS: Preferential metabolic activation of N-nitrosopiperidine as compared to its structural homologue N-nitrosopyrrolidine by rat nasal mucosal microsomes, Chem Res Toxicol 2003, 16:1298-1305
• Wang M, Cheng G, Sturla SJ, Shi Y, McIntee EJ, Villalta PW, Upadhyaya P, Hecht SS: Identification of adducts formed by pyridyloxobutylation of deoxyguanosine and DNA by 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone, a chemically activated form of tobacco specific carcinogens, Chem Res Toxicol 2003, 16:616-626
• Upadhyaya P, Sturla SJ, Tretyakova N, Ziegel R, Villalta PW, Wang M, Hecht SS: Identification of adducts produced by the reaction of 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanol with deoxyguanosine and DNA, Chem Res Toxicol 2003, 16:180-190
• Jalas JR, McIntee EJ, Kenney PM, Upadhyaya P, Peterson LA, Hecht SS: Stereospecific deuterium substitution attenuates the tumorigenicity and metabolism of the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), Chem Res Toxicol 2003, 16:794-806
• Jalas JR, Hecht SS: Synthesis of stereospecifically deuterated 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) iastereomers and metabolism by A/J mouse lung microsomes and cytochrome p450 2A5, Chem Res Toxicol 2003, 16:782-793
• Hecht SS, Chen M, Yagi H, Jerina DM, Carmella SG: r-1,t-2,3,c-4-Tetrahydroxy-1,2,3,4-tetrahydrophenanthrene in human urine: a potential biomarker for assessing polycyclic aromatic hydrocarbon metabolic activation, Cancer Epidemiol Biomarkers Prev 2003, 12:1501-1508
• Hatsukami DK, Hecht SS, Hennrikus DJ, Joseph AM, Pentel PR: Biomarkers of tobacco exposure or harm: application to clinical and epidemiological studies. 25-26 October 2001, Minneapolis, Minnesota, Nicotine Tob Res 2003, 5:387-396
• Hatsukami DK, Edmonds A, Schulte S, Jensen J, Le CT, Losey L, Carmella SG, Hecht SS: Preliminary study on reducing oral moist snuff use, Drug Alcohol Depend 2003, 70:215-220
• Cheng G, Shi Y, Sturla SJ, Jalas JR, McIntee EJ, Villalta PW, Wang M, Hecht SS: Reactions of formaldehyde plus acetaldehyde with deoxyguanosine and DNA: formation of cyclic deoxyguanosine adducts and formaldehyde cross-links, Chem Res Toxicol 2003, 16:145-152
• Carmella SG, Han S, Fristad A, Yang Y, Hecht SS: Analysis of total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) in human urine, Cancer Epidemiol Biomarkers Prev 2003, 12:1257-1261
• Boysen G, Kenney PM, Upadhyaya P, Wang M, Hecht SS: Effects of benzyl isothiocyanate and 2-phenethyl isothiocyanate on benzo[a]pyrene and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone metabolism in F-344 rats, Carcinogenesis 2003, 24:517-525
• Boysen G, Hecht SS: Analysis of DNA and protein adducts of benzo[a]pyrene in human tissues using structure-specific methods, Mutat Res 2003, 543:17-30
• Anderson KE, Kliris J, Murphy L, Carmella SG, Han S, Link C, Bliss RL, Puumala S, Murphy SE, Hecht SS: Metabolites of a tobacco-specific lung carcinogen in nonsmoking casino patrons, Cancer Epidemiol Biomarkers Prev 2003, 12:1544-1546